吴长艾
个人简介
吴长艾，女，山东巨野人。2004年获得山东农业大学理学博士学位，至今在山东农业大学生命科学学院工作。生物化学与分子生物学国家级教学团队骨干。承担本科生《生物化学A》、《基础生物化学B》和《基因工程》；研究生《基因工程原理和技术》等课程的教学工作。主要从事粮食作物谷子耐盐、抗旱、高产、优质等重要性状控制基因的克隆、鉴定和遗传机制解析，进行谷子种质资源的耐盐性鉴定和耐盐抗旱抗除草剂谷子新种质创制。先后主持并承担转基因生物新品种培育科技重大专项、国家重点研发计划子课题、国家自然科学基金、山东省自然科学基金重大基础研究计划等课题14项。获得国家发明专利10件，山东省自然科学二等奖1项。在《Molecular Plant》、 《Cell reports》、《Plant Journal》和《作物学报》等国内外著名学术刊物上发表研究论文60余篇。
教育教学工作
承担本科生《基础生物化学》，《基础生物化学实验》；研究生《基因工程原理与技术》、《基因表达调控》等课程的教学工作。
研究方向
1. 作物耐盐、抗旱、促生新型激素鉴定、遗传机制解析及应用评价。
2. 谷子穗、籽粒等重要产量性状形成的遗传机制解析。
3. 谷子耐盐碱、抗旱、高产、优质新种质创制。
科研项目
1. 国家自然科学基金《转录辅助因子SiTAF10调控谷子耐盐性的分子机理》，2025.01-2028.12，主持
2. 国家自然科学基金《SRG1小肽提高谷子耐盐性的分子机制和调控网络解析》，2023.01-2025.12，主持
3. 国家重点研发计划《作物耐盐碱高效高产基因资源挖掘与利用》-课题四子课题《谷子耐盐性鉴定和耐盐新种质的创制》，2022.12-2027.11，主持
4. 中央引导地方科技发展项目《新型植物激素的挖掘、生理活性和作用机理》，2021.11-2023.12，主持
5. 山东省农业良种工程子课题《谷子高效育种技术研究》2018.07-2024.12，主持
6. 国家重点研发计划《杂粮作物抗逆和品质形成与调控》-课题四子课题《谷子盐胁迫响应SiPLATZ转录因子的作用机理研究》，2018.07-2022.12，主持
7. 山东省自然科学基金重大基础研究项目《利用CRISPR/Cas9 基因组编辑技术创制玉米抗旱耐盐新种质》，2018.06-2020.12，主持
8. 山东省自然科学基金《棉花新型转录调节因子GhPLATZ1的功能和作用机理研究》，2015-2017，主持
9. 973前期《玉米高温干旱抗性关键 microRNA基因的分离及功能鉴定》，2014-2016，第二位
10. 国家自然科学基金《棉花盐诱导的4个海藻糖6-磷酸合酶和6个LEA基因的生理功能及其应用研究》2011-2013，主持
11. 转基因生物新品种培育科技重大专项《棉花抗旱、耐盐和低温重要新基因的克隆和功能验证》，2009-2012，主持
12. 国家自然科学基金《五个拟南芥盐和干旱诱导的miRNAs的功能鉴定及调控途径研究》，2010-2012，主持
13. 国家自然科学基金《水稻干旱、盐胁迫相关microRNAs的分离和表达调控分析》2006-2008，主持
14. 教育部“长江学者创新团队发展计划”《作物高产优质的生理与分子基础》子课题，2007-2009，主持
发表论文
1. Lei Zhang, Zipeng Yu, Yang Xu, Miao Yu, Yue Ren, Shizhong Zhang, Guodong Yang, Jinguang Huang, Kang Yan, Chengchao Zheng*, Changai Wu* (2021) Regulation of the stability and ABA import activity of NRT1.2/NPF4.6 by CEPR2-mediated phosphorylation in Arabidopsis. Molecular Plant, 14: 633-646
2. Xiaoxiao Liu, Lei Zhang, Zeqi Zhao, Yue Zheng, Yue Ren, Xiaomin Zhao, Shizhong Zhang, Guodong Yang, Jinguang Huang, Kang Yan, Chunlong Li*, Chengchao Zheng*, Changai Wu* (2025) Regulation of the non-selective Na+ importer capacity of NRT1.2/NPF4.6/AIT1 by SOS2-mediated phosphorylation in Arabidopsis. Cell Reports, 44: 115729
3. Miao Liu, Chunyan Wang, Zhen Ji, Junyao Lu, Lei Zhang, Chunlong Li, Jinguang Huang, Guodong Yang, Kang Yan, Shizhong Zhang, Chengchao Zheng*, Changai Wu* (2023) Regulation of drought tolerance in Arabidopsis involves the PLATZ4-mediated transcriptional repression of plasma membrane aquaporin PIP2;8. Plant Journal, 115: 434–451
4. Zhen Wang, Hao Zheng, Jinguang Huan, Guodong Yang, Kang Yan, Shizhong Zhang, Changai Wu*, Chengchao Zheng* (2022) DEMETHYLATION REGULATOR 1 regulates DNA demethylation of the nuclear and mitochondrial genomes. Journal of Integrative Plant Biology, 64: 2344–2360
5. Chunyan Wang, Tongtong Wang, Miao Liu, Shizhong Zhang, Changai Wu* (2024) Small peptide SiDVL/RTFLs from foxtail millet inhibit root growth through repressing auxin signaling in transgenic Arabidopsis. Plant Cell Reports, 43: 268.
6. Shenghui Xiao, Yiman Wan, Yue Zheng, Yongdong Wang, Jiayin Fan, Qian Xu, Zheng Gao*, Changai Wu* (2023) Halomonas ventosae JPT10 promotes salt tolerance in foxtail millet (Setaria italica) by affecting the levels of multiple antioxidants and phytohormones. Plant-Environment Interactions, 4, 275–290
7. Changai Wu*, Meng Zhang, Yifan Liang, Lei Zhang, Xianmin Diao (2023) Salt stress responses in foxtail millet: Physiological and molecular regulation. Crop Journal, 11: 1011-1021
8. Shenghui Xiao, Linlin Zhang, Yiman Wan, Sha Tang, Yi Sui, Yichao Bai, Yan Wang, Miao Liu, Jiayin Fan, Shizhong Zhang, Jinguang Huang, Guodong Yang, Kang Yan, Xianmin Diao*, Chengchao Zheng*, Changai Wu* (2023) SiPLATZ12 transcription factor negatively regulates salt tolerance in foxtail millet (Setaria italica) by suppressing the expression of SiNHX, SiCBL and SiSOS genes. Environmental and Experimental Botany, 213, 105417
9. Shenghui Xiao, Yiman Wan, Shiming Guo, Jiayin Fan, Qing Lin, Chengchao Zheng, Changai Wu* (2023) Transcription factor SiDi19-3 enhances salt tolerance of foxtail millet and Arabidopsis. International Journal of Molecular Sciences, 24, 2592
10. Lei Zhang, Yue Ren, Qian Xu, Yiman Wan, Shizhong Zhang, Guodong Yang, Jinguang Huang, Kang Yan, Chengchao Zheng, Changai Wu* (2021) SiCEP3, a C-terminally encoded peptide from Setaria italica, promotes ABA import and signaling. Journal of Experimental Botany, 72 (18): 6260–6273.
11. Shasha Liu, Rui Yang, Miao Liu, Shizhong Zhang, Kang Yan, Guodong Yang, Jinguang Huang, Chengchao Zheng, Changai Wu* (2020) PLATZ2 negatively regulates salt tolerance in Arabidopsis seedlings by directly suppressing the expression of the CBL4/SOS3 and CBL10/SCaBP8 genes. Journal of Experimental Botany, 71 (18): 5589–5602.
12. Zipeng Yu, Di Zhang, Yang Xu, Songsong Jin, Lei Zhang, Shizhong Zhang, Guodong Yang, Jinguang Huang, Kang Yan, Changai Wu*, Chengchao Zheng (2019) CEPR2 phosphorylates and accelerates the degradation of PYR/PYLs in Arabidopsis. Journal of Experimental Botany, 70 (19): 5457–5469.
13. Zipeng Yu, Yang Xu, Lin Liu, Yarong Guo, Xisen Yuan, Xinyu Man, Chang Liu, Guodong Yang, Jinguang Huang, Kang Yan, Chengchao Zheng, Changai Wu,* and Shizhong Zhang* (2019) The Importance of Conserved Serine for C-Terminally Encoded Peptides Function Exertion in Apple. International Journal of Molecular Sciences, 20: 775.
14. Shicai Zhang, Rui Yang, Yanqing Huo, Shasha Liu, Guodong Yang, Jinguang Huang, Chengchao Zheng, Changai Wu* (2018) Expression of cotton PLATZ1 in transgenic Arabidopsis reduces sensitivity to osmotic and salt stress for germination and seedling establishment associated with modification of the abscisic acid, gibberellin, and ethylene signaling pathways. BMC Plant Biology, 18: 218
15. Yumeng Chen, Yan Wang, Jinguang Huang, Chengchao Zheng, Congxi Cai, Qiaomei Wang, Changai Wu* (2017) Salt and methyl jasmonate aggravate growth inhibition and senescence in Arabidopsis seedlings via the JA signaling pathway. Plant Science 261: 1-9.
16. Caili Wang, Shicai Zhang, Shengdong Qi, Chengchao Zheng, Changai Wu* (2016) Delayed germination of Arabidopsis seeds under chilling stress by overexpressing an abiotic stress inducible GhTPS11. GENE, 575: 206-212.
17. Changtian Chen, Changai Wu, Jiaming Miao, Yunxue Lei, Dongxiao Zhao, Dan Sun, Guodong Yang, Jinguang Huang and Chengchao Zheng. (2014) Arabidopsis SAG protein containing the MDN1 domain participates in seed germination and seedling development by negatively regulating ABI3 and ABI5. Journal of Experimental Botany, 65(1): 35-45.
18. Nana Ma, Hailong Feng, Xia Meng, Dong Li, Dongyue Yang, Changai Wu*, Qingwei Meng*. (2014) Overexpression of tomato SlNAC1 transcription factor alters fruit pigmentation and softening. BMC Plant Biology, 14:351.
19. Yunxue Lei, Yaru Fu, Changtian Chen, Jinguang Huang, Changai Wu*. (2014) Overexpression of Arabidopsis wali7 domain-containing protein ASR produces auxin-mediated short-root phenotype. Journal of Plant Growth Regulation, 33:355-363.
20. Yanjie Li, Yi Fang, Yaru Fu, Jinguang Huang, Changai Wu*, Chengchao Zheng*. (2013) NFYA1 is involved in regulation of postgermination growth arrest under salt stress in Arabidopsis.PLoS One, 8(4): e61289.
21. Peng Liu, Kang Yan, YunXue Lei, Rui Xu, YueMin Zhang, Guodong Yang, Jinguang Huang, Changai Wu*, Chengchao Zheng*. (2013) Transcript profiling of microRNAs during the early development of the maize brace root via Solexa sequencing. Genomics, 101: 149-156
22. Wei Lu, Xiaoli Tang, Yanqing Huo, Rui Xu, Shengdong Qi, Jingguang Huang, Chengchao Zheng, Changai Wu*. (2012) Identification and characterization of fructose 1,6-bisphosphate aldolase genes in Arabidopsis reveals a gene family with diverse responses to abiotic stresses. Gene, 503: 65-74.
23. Gang Wang, Qingguo Zhu, Qingwei Meng, Changai Wu*. (2012) Transcript profiling during salt stress of young cotton (Gossypium hirsutum) seedlings via Solexa sequencing. Acta Physiologiae Plantarum, 34:107-115.
24. Yanjie Li, Yaru Fu, Jingguang Huang, Changai Wu*, Chengchao Zheng*. (2011) Transcript profiling during the early development of the maize brace root via Solexa sequencing. The FEBS Journal, 278: 156-166.
25. Ruirui Xu, Shengdong Qi, Longtao Lu, Changtian Chen, Changai Wu*, Chengchao Zheng*. (2011) A DExD/H box RNA helicase is important for potassium deprivation responses and tolerance in Arabidopsis thaliana. The FEBS Journal, 278(13): 2293-2306.
26. Jing Shi, Liang Zhang, Hailong An, Changai Wu*, Xingqing Guo*. (2011) GhMPK16, a novel stress-responsive group D MAPK gene from cotton, is involved in disease resistance and drought sensitivity. BMC Molecular Biology, 12:22.
27. Yanjie Li, Yaru Fu, Lusha Ji, Changai Wu*, Chengchao Zheng*. (2010). Characterization and expression analysis of the Arabidopsis mir169 family. Plant Science, 178: 271-280.
28. Pei Liu, Guodong Yang, Hong Li, Changai Wu*. (2010) Overexpression of NHX1s in transgenic Arabidopsis enhances photoprotection capacity in high salinity and drought conditions. Acta Physiologiae Plantarum, 32: 81-90.
29. Hanhua Liu, Xin Tian, Yanjie Li, Changai Wu*, Chengchao Zheng*. (2008) Microarray-based Analysis of Stress-regulated microRNAs in Arabidopsis thaliana. RNA, 14: 836-843.
30. Changai Wu, Guodong Yang, Qingwei Meng, Chengchao Zheng. (2004) The cotton GhNHX1 gene encoding a novel putative tonoplast Na+/H+ antiporter plays an important role in salt stress. Plant & Cell Physiology, 45: 600-607.
获得专利
1. 一种调控谷子生长发育的SiPLATZ12基因及其应用，专利号：ZL 2021 1 1513791.4，吴长艾，肖圣慧，范佳音，郑成超。
2. 一种提高谷子抗旱性和耐盐性的SiTAF10基因及其应用，专利号：ZL202310094401.7，吴长艾，白依超，张萌，肖圣慧，郑成超。
3. 布洛芬在提高植物耐盐性中的应用，专利号：ZL202011257582.3，吴长艾，肖圣慧，万夷曼，王琰，白依超，郑成超。
4. 菲尼酮在提高植物耐盐性中的应用，专利号：ZL202011257583.8，吴长艾，肖圣慧，万夷曼，王琰，白依超，郑成超。
5. 8-甲氧基山奈酚在制备促进植物生长、提高植物耐盐性的制剂中的应用，专利号：ZL202010854404.2，吴长艾，肖圣慧，万夷曼，白依超，郑成超。
6. 芹菜素在制备促进植物生长、提高植物耐盐性的制剂中的应用，专利号：ZL202010854231.4，吴长艾，肖圣慧，万夷曼，白依超，郑成超。
7. 棉花Na+/H+反向转运蛋白基因及其应用，专利号：ZL02151530.1， 郑成超，吴长艾，杨国栋。
获奖情况
1. 2023年获山东省研究生创新成果奖《番茄EILs介导的光保护途径》（指导教师）
2. 2021年获山东省研究生优秀成果奖二等奖《CEP小肽及其受体CEPR参与植物生长和ABA响应的机理研究》（指导教师）
3. 2021年获山东农业大学“十佳”研究生导学团队
4. 2020年获山东省优秀博士毕业论文《CEPR2调控拟南芥ABA响应的机理研究》（指导教师）
5. 2015年获山东省自然科学二等奖，第二位
6. 2012年获得山东农业大学“青年岗位能手”荣誉称号
7. 2011年获得山东农业大学“巾帼建功先进个人”荣誉称号
8. 2009年获得山东农业大学优秀学士学位论文（指导教师）
9. 2004年获得山东省优秀博士毕业生
学术兼职
1. Frontiers in Plant Science 编委。
2. 国际期刊审稿专家。
3. 山东省植物生物学与分子生物学理事。
联系方式
通讯地址：山东省泰安市岱宗大街61号
联系电话: 18605383306 E-mail: cawu@sdau.edu.cn
联系地址：国重楼#618
